@article{Sanders:2017:nar/gkx074,
author = {Sanders, K and Lin, C-L and Smith, AJ and Cronin, N and Fisher, G and Eftychidis, V and McGlynn, P and Savery, NJ and Wigley, DB and Dillingham, MS},
doi = {nar/gkx074},
journal = {Nucleic Acids Research},
pages = {3875--3887},
title = {The structure and function of an RNA polymerase interaction domain in the PcrA/UvrD helicase},
url = {http://dx.doi.org/10.1093/nar/gkx074},
volume = {45},
year = {2017}
}

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TY  - JOUR
AB  - The PcrA/UvrD helicase functions in multiple pathways that promote bacterial genome stability including the suppression of conflicts between replication and transcription and facilitating the repair of transcribed DNA. The reported ability of PcrA/UvrD to bind and backtrack RNA polymerase (1,2) might be relevant to these functions, but the structural basis for this activity is poorly understood. In this work, we define a minimal RNA polymerase interaction domain in PcrA, and report its crystal structure at 1.5 Å resolution. The domain adopts a Tudor-like fold that is similar to other RNA polymerase interaction domains, including that of the prototype transcription-repair coupling factor Mfd. Removal or mutation of the interaction domain reduces the ability of PcrA/UvrD to interact with and to remodel RNA polymerase complexes in vitro. The implications of this work for our understanding of the role of PcrA/UvrD at the interface of DNA replication, transcription and repair are discussed.
AU  - Sanders,K
AU  - Lin,C-L
AU  - Smith,AJ
AU  - Cronin,N
AU  - Fisher,G
AU  - Eftychidis,V
AU  - McGlynn,P
AU  - Savery,NJ
AU  - Wigley,DB
AU  - Dillingham,MS
DO  - nar/gkx074
EP  - 3887
PY  - 2017///
SN  - 0305-1048
SP  - 3875
TI  - The structure and function of an RNA polymerase interaction domain in the PcrA/UvrD helicase
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gkx074
UR  - http://hdl.handle.net/10044/1/45484
VL  - 45
ER  - 

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