@article{Wong:2011:10.1371/journal.pone.0026130,
author = {Wong, EEH and Li, M-S and Kroll, JS and Hibberd, ML and Langford, PR},
doi = {10.1371/journal.pone.0026130},
journal = {PLOS One},
pages = {e26130--e26130},
title = {Genome wide expression profiling reveals suppression of host defence responses during colonisation by Neisseria meningitidis but not N. lactamica},
url = {http://dx.doi.org/10.1371/journal.pone.0026130},
volume = {6},
year = {2011}
}

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TY  - JOUR
AB  - Both Neisseria meningitidis and the closely related bacterium Neisseria lactamica colonise human nasopharyngeal mucosal surface, but only N. meningitidis invades the bloodstream to cause potentially life-threatening meningitis and septicaemia. We have hypothesised that the two neisserial species differentially modulate host respiratory epithelial cell gene expression reflecting their disease potential. Confluent monolayers of 16HBE14 human bronchial epithelial cells were exposed to live and/or dead N. meningitidis (including capsule and pili mutants) and N. lactamica, and their transcriptomes were compared using whole genome microarrays. Changes in expression of selected genes were subsequently validated using Q-RT-PCR and ELISAs. Live N. meningitidis and N. lactamica induced genes involved in host energy production processes suggesting that both bacterial species utilise host resources. N. meningitidis infection was associated with down-regulation of host defence genes. N. lactamica, relative to N. meningitidis, initiates up-regulation of proinflammatory genes. Bacterial secreted proteins alone induced some of the changes observed. The results suggest N. meningitidis and N. lactamica differentially regulate host respiratory epithelial cell gene expression through colonisation and/or protein secretion, and that this may contribute to subsequent clinical outcomes associated with these bacteria.
AU  - Wong,EEH
AU  - Li,M-S
AU  - Kroll,JS
AU  - Hibberd,ML
AU  - Langford,PR
DO  - 10.1371/journal.pone.0026130
EP  - 26130
PY  - 2011///
SN  - 1932-6203
SP  - 26130
TI  - Genome wide expression profiling reveals suppression of host defence responses during colonisation by Neisseria meningitidis but not N. lactamica
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0026130
UR  - http://www.ncbi.nlm.nih.gov/pubmed/22028815
UR  - http://www.plosone.org/article/info%3Adoi/10.1371/journal.pone.0026130
UR  - http://hdl.handle.net/10044/1/27343
VL  - 6
ER  - 

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