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• Journal article
  Brenlla A, Markiewicz RP, Rueda D, Romano LJet al., 2014,

  Nucleotide selection by the Y-family DNA polymerase Dpo4 involves template translocation and misalignment

  , Nucleic Acids Research, Vol: 42, Pages: 2555-2563, ISSN: 0305-1048

  Y-family DNA polymerases play a crucial role in translesion DNA synthesis. Here, we have characterized the binding kinetics and conformational dynamics of the Y-family polymerase Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) using single-molecule fluorescence. We find that in the absence of dNTPs, the binary complex shuttles between two different conformations within ∼1 s. These data are consistent with prior crystal structures in which the nucleotide binding site is either occupied by the terminal base pair (preinsertion conformation) or empty following Dpo4 translocation by 1 base pair (insertion conformation). Most interestingly, on dNTP binding, only the insertion conformation is observed and the correct dNTP stabilizes this complex compared with the binary complex, whereas incorrect dNTPs destabilize it. However, if the n+1 template base is complementary to the incoming dNTP, a structure consistent with a misaligned template conformation is observed, in which the template base at the n position loops out. This structure provides evidence for a Dpo4 mutagenesis pathway involving a transient misalignment mechanism.

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• Journal article
  Macdonald DC, Singh H, Whelan MA, Escors D, Arce F, Bottoms SE, Barclay WS, Maini M, Collins MK, Rosenberg WMCet al., 2014,

  Harnessing alveolar macrophages for sustained mucosal T-cell recall confers long-term protection to mice against lethal influenza challenge without clinical disease

  , MUCOSAL IMMUNOLOGY, Vol: 7, Pages: 89-100, ISSN: 1933-0219
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  • Citations: 15
• Journal article
  Paudel B, Rueda D, 2014,

  RNA Folding Dynamics Using Laser-Assisted Single-Molecule Refolding

  , RNA FOLDING: METHODS AND PROTOCOLS, Vol: 1086, Pages: 289-307, ISSN: 1064-3745
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  • Citations: 3
• Journal article
  Karunatilaka KS, Rueda D, 2014,

  Post-transcriptional modifications modulate conformational dynamics in human U2-U6 snRNA complex

  , RNA, Vol: 20, Pages: 16-23, ISSN: 1355-8382
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  • Citations: 19
• Journal article
  Mundigala H, Michaux JB, Feig AL, Ennifar E, Rueda Det al., 2014,

  HIV-1 DIS stem loop forms an obligatory bent kissing intermediate in the dimerization pathway

  , NUCLEIC ACIDS RESEARCH, Vol: 42, Pages: 7281-7289, ISSN: 0305-1048
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  • Citations: 9
• Journal article
  Hill ER, Koganti S, Zhi J, Megyola C, Freeman AF, Palendira U, Tangye SG, Farrell PJ, Bhaduri-McIntosha Set al., 2013,

  Signal Transducer and Activator of Transcription 3 Limits Epstein-Barr Virus Lytic Activation in B Lymphocytes

  , JOURNAL OF VIROLOGY, Vol: 87, Pages: 11438-11446, ISSN: 0022-538X
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  • Citations: 36
• Journal article
  Goujon C, Moncorge O, Bauby H, Doyle T, Ward CC, Schaller T, Hue S, Barclay WS, Schulz R, Malim MHet al., 2013,

  Human MX2 is an interferon-induced post-entry inhibitor of HIV-1 infection

  , NATURE, Vol: 502, Pages: 559-+, ISSN: 0028-0836
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  • Citations: 406
• Journal article
  Blumenkrantz D, Roberts KL, Shelton H, Lycett S, Barclay WSet al., 2013,

  The Short Stalk Length of Highly Pathogenic Avian Influenza H5N1 Virus Neuraminidase Limits Transmission of Pandemic H1N1 Virus in Ferrets

  , JOURNAL OF VIROLOGY, Vol: 87, Pages: 10539-10551, ISSN: 0022-538X
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  • Citations: 54
• Journal article
  Long JS, Howard WA, Nunez A, Moncorge O, Lycett S, Banks J, Barclay WSet al., 2013,

  The Effect of the PB2 Mutation 627K on Highly Pathogenic H5N1 Avian Influenza Virus Is Dependent on the Virus Lineage

  , JOURNAL OF VIROLOGY, Vol: 87, Pages: 9983-9996, ISSN: 0022-538X
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  • Citations: 40
• Journal article
  Vrtis KB, Markiewicz RP, Romano LJ, Rueda Det al., 2013,

  Carcinogenic adducts induce distinct DNA polymerase binding orientations

  , NUCLEIC ACIDS RESEARCH, Vol: 41, Pages: 7843-7853, ISSN: 0305-1048
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  • Citations: 17

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