@article{Gonzalo:2022:10.1016/j.cmi.2022.10.017,
author = {Gonzalo, X and Yrah, S and Broda, A and Laurenson, I and Claxton, P and Kostrzewa, M and Drobniewski, F and Larrouy-Maumus, G},
doi = {10.1016/j.cmi.2022.10.017},
journal = {Clin Microbiol Infect},
title = {Performance of lipid fingerprint by routine matrix-assisted laser desorption/ionization time of flight for the diagnosis of Mycobacterium tuberculosis complex species.},
url = {http://dx.doi.org/10.1016/j.cmi.2022.10.017},
year = {2022}
}

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TY  - JOUR
AB  - OBJECTIVES: Rapid detection of bacterial pathogens at species and sub-species levels is crucial for appropriate treatment, infection control, and public health management. Currently, one of the challenges in clinical microbiology is the discrimination of mycobacterial sub-species within the M. tuberculosis complex (MTBC). Our objective was to evaluate the ability of a biosafe mycobacterial lipid-based approach to identify MTBC cultures and sub-species. METHODS: A blinded study was conducted using 90 mycobacterial clinical isolate strains comprising MTBC strains sub-cultured in Middlebrook 7H11 medium supplemented with 10% oleic-acid, dextrose, catalase growth supplement and incubated for up to 6 weeks at 37°C and using the following seven reference strains (M. tuberculosis H37Rv, M canettii, M. africanum, M. pinnipedii, M. caprae, M. bovis, and M. bovis BCG) grown under the same conditions, to set the reference lipid database and test it against the 90 MTBC clinical isolates. Cultured mycobacteria were heat-inactivated and loaded onto the matrix-assisted laser desorption/ionization target followed by the addition of the matrix. Acquisition of the data was performed using the positive ion mode. RESULTS: Based on the identification of clear and defined lipid signatures from the seven reference strains, the method that we developed was fast (<10 minutes) and produced interpretable profiles for all but four isolates, caused by poor ionization giving an n = 86 with interpretable spectra. The sensitivity and specificity of the matrix-assisted laser desorption/ionization time of flight were 94.4 (95% CI, 86.4-98.5) and 94.4 (95% CI, 72.7-99.9), respectively. CONCLUSIONS: Mycobacterial lipid profiling provides a means of rapid, safe, and accurate discrimination of species within the MTBC.
AU  - Gonzalo,X
AU  - Yrah,S
AU  - Broda,A
AU  - Laurenson,I
AU  - Claxton,P
AU  - Kostrzewa,M
AU  - Drobniewski,F
AU  - Larrouy-Maumus,G
DO  - 10.1016/j.cmi.2022.10.017
PY  - 2022///
TI  - Performance of lipid fingerprint by routine matrix-assisted laser desorption/ionization time of flight for the diagnosis of Mycobacterium tuberculosis complex species.
T2  - Clin Microbiol Infect
UR  - http://dx.doi.org/10.1016/j.cmi.2022.10.017
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36270589
ER  - 

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